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Journal: Biomedicines
Article Title: Intraepithelial Lymphocytes and LAIR1 Expression in Celiac Disease
doi: 10.3390/biomedicines13102526
Figure Lengend Snippet: Immunophenotype characterization of IELs in the intestinal mucosa control. Most IELs expressed TCRβ chains. Occasionally, CD56 + IELs were found, as well as TCRδ chain-positive IELs (i.e., TCRγδ + IELs). TCRB, TCR beta (β); TCRD, TCR delta (δ). TCR delta was analyzed using two different clones: H-41 (Santa Cruz, sc-100289) and E2E9T (Cell Signaling, TRDC/TCRδ (E2E9T) XP® Rabbit mAb #55750). Original magnification 200× and 400×.
Article Snippet:
Techniques: Control, Clone Assay
Journal: bioRxiv
Article Title: Endocrine therapy induces oxidative stress in ER+ breast cancer that sensitizes persister cells to ferroptosis
doi: 10.1101/2025.07.18.665614
Figure Lengend Snippet: A) Volcano plot of lipids differentially abundant in T47D cells treated ± 1 μM fulvestrant for 7 d. Highlighted are lipids with p<0.05. B-C) Pearson correlation plots of lipid carbon number (B) and degree of desaturation (C) with log2(fold change) from (A). Only differential lipids with p<0.05 are included. p is calculated by two-tailed Pearson correlation test. C) Relative abundances of PE PUFA lipid species from (A) that were found with p<0.05 with 5% FDR. D) Relative viability of T47D cells treated with +/− RSL3 and ±adrenic acid for 3 d. Viability was assessed by SRB. E) Flow cytometry analysis of T47D cells treated with RSL3 and adrenic acid for 4 h before staining with BODIPY C11 and flow cytometry. F) Relative viability plot of T47D cells pre-treated with fulvestrant for 7 d before additional treatment with RSL3 and PRGL493. G) Viability plots of cells pre-treated with 1 μM fulvestrant before subsequent treatment +/− RSL3, SC26196, and CP-24879.
Article Snippet: Cells were treated as indicated with fulvestrant (Selleck Chemicals #S1191), 17β-estradiol (E2; Sigma #E2758), N-acetyl-L-cysteine (Sigma #A9165), reduced L-glutathione (GSH; Sigma #G6013), RSL3 (Cayman Chemical #19288), ferrostatin-1 (Sigma #SML0583), deferoxamine (Sigma #D9533),
Techniques: Two Tailed Test, Flow Cytometry, Staining